LOCUS       40924_48643        8266 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector P{attP.w+.attP}, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8266)
  AUTHORS   Bateman JR, Wu CT.
  TITLE     A simple polymerase chain reaction-based method for the construction
            of recombinase-mediated cassette exchange donor vectors
  JOURNAL   Genetics 180 (3), 1763-1766 (2008)
  PUBMED    18791223
REFERENCE   2  (bases 1 to 8266)
  AUTHORS   Bateman JR.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2008) Genetics, Harvard Medical School, 77 Avenue 
            Louis Pasteur, Boston, MA 02115, USA
REFERENCE   3  (bases 1 to 8266)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8266)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "2008"; volume: "180"; issue: "3"; pages: "1763-1766"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-MAY-2008) Genetics, Harvard Medical School, 77 Avenue Louis 
            Pasteur, Boston, MA 02115, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    365..464
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     misc_feature    549..4569
                     /label=mini-white dominant eye marker
                     /note="mini-white dominant eye marker"
     CDS             join(1035..1106,1501..1774,1849..2503,2565..2880,3101..3232,
                     3303..3917)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    complement(4632..4731)
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     misc_feature    complement(4921..5506)
                     /label=P element 5' end
     rep_origin      complement(5741..6329)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6503..7360)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7361..7465)
                     /label=AmpR promoter
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttc
      241 tgcatctctc cggatccact agtaacggcc gccagtgtgc tggaattcgg cttcgactag
      301 tactgacgga cacaccgaag ccccggcggc aaccctcagc ggatgccccg gggcttcacg
      361 ttttcccagg tcagaagcgg ttttcgggag tagtgcccca actggggtaa cctttgagtt
      421 ctctcagttg ggggcgtagg gtcgccgaca tgacacaagg ggttgtgacc ggggtggaca
      481 cgtacgcggg tgcttacgac cgtcagtcgc gcgagcgcga ctagtacaag ccgaattctg
      541 cagatcctta gtatgtatgt aagttaataa aacccttttt tggagaatgt agatttaaaa
      601 aaacatattt tttttttatt ttttactgca ctggacatca ttgaacttat ctgatcagtt
      661 ttaaatttac ttcgatccaa gggtatttga agtaccaggt tctttcgatt acctctcact
      721 caaaatgaca ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat
      781 cgccgtctct ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttaccta
      841 gcgtcaatgt ccgccttcag ttgcactttg tcagcggttt cgtgacgaag ctccaagcgg
      901 tttacgccat caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg
      961 tttgtttttt gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt
     1021 gaaaaatccc ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac
     1081 acccatctgc cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac
     1141 ttgatctata ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc
     1201 acaaaaatcc caaaccgcaa tcgcacaaac aaatagtgac acgaaacaga ttattctggt
     1261 agctgtgctc gctatataag acaattttta agatcatatc atgatcaaga catctaaagg
     1321 cattcatttt cgactacatt cttttttaca aaaaatataa caaccagata ttttaagctg
     1381 atcctagatg cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcgtttt
     1441 gttcggggtt agatgagcat aacgcttgta gttgatattt gagatcccct atcattgcag
     1501 ggtgacagcg gagcggcttc gcagagctgc attaaccagg gcttcgggca ggccaaaaac
     1561 tacggcacgc tcctgccacc cagtccgccg gaggactccg gttcagggag cggccaacta
     1621 gccgagaacc tcacctatgc ctggcacaat atggacatct ttggggcggt caatcagccg
     1681 ggctccggat ggcggcagct ggtcaaccgg acacgcggac tattctgcaa cgagcgacac
     1741 ataccggcgc ccaggaaaca tttgctcaag aacggtgagt ttctattcgc agtcggctga
     1801 tctgtgtgaa atcttaataa agggtccaat taccaatttg aaactcagtt tgcggcgtgg
     1861 cctatccggg cgaacttttg gccgtgatgg gcagttccgg tgccggaaag acgaccctgc
     1921 tgaatgccct tgcctttcga tcgccgcagg gcatccaagt atcgccatcc gggatgcgac
     1981 tgctcaatgg ccaacctgtg gacgccaagg agatgcaggc caggtgcgcc tatgtccagc
     2041 aggatgacct ctttatcggc tccctaacgg ccagggaaca cctgattttc caggccatgg
     2101 tgcggatgcc acgacatctg acctatcggc agcgagtggc ccgcgtggat caggtgatcc
     2161 aggagctttc gctcagcaaa tgtcagcaca cgatcatcgg tgtgcccggc agggtgaaag
     2221 gtctgtccgg cggagaaagg aagcgtctgg cattcgcctc cgaggcacta accgatccgc
     2281 cgcttctgat ctgcgatgag cccacctccg gactggactc atttaccgcc cacagcgtcg
     2341 tccaggtgct gaagaagctg tcgcagaagg gcaagaccgt catcctgacc attcatcagc
     2401 cgtcttccga gctgtttgag ctctttgaca agatccttct gatggccgag ggcagggtag
     2461 ctttcttggg cactcccagc gaagccgtcg acttcttttc ctagtgagtt cgatgtgttt
     2521 attaagggta tctagcatta cattacatct caactcctat ccagcgtggg tgcccagtgt
     2581 cctaccaact acaatccggc ggacttttac gtacaggtgt tggccgttgt gcccggacgg
     2641 gagatcgagt cccgtgatcg gatcgccaag atatgcgaca attttgctat tagcaaagta
     2701 gcccgggata tggagcagtt gttggccacc aaaaatttgg agaagccact ggagcagccg
     2761 gagaatgggt acacctacaa ggccacctgg ttcatgcagt tccgggcggt cctgtggcga
     2821 tcctggctgt cggtgctcaa ggaaccactc ctcgtaaaag tgcgacttat tcagacaacg
     2881 gtgagtggtt ccagtggaaa caaatgatat aacgcttaca attcttggaa acaaattcgc
     2941 tagattttag ttagaattgc ctgattccac acccttctta gtttttttca atgagatgta
     3001 tagtttatag ttttgcagaa aataaataaa tttcatttaa ctcgcgaaca tgttgaagat
     3061 atgaatatta atgagatgcg agtaacattt taatttgcag atggttgcca tcttgattgg
     3121 cctcatcttt ttgggccaac aactcacgca agtgggcgtg atgaatatca acggagccat
     3181 cttcctcttc ctgaccaaca tgacctttca aaacgtcttt gccacgataa atgtaagtct
     3241 tgtttagaat acatttgcat attaataatt tactaacttt ctaatgaatc gattcgattt
     3301 aggtgttcac ctcagagctg ccagttttta tgagggaggc ccgaagtcga ctttatcgct
     3361 gtgacacata ctttctgggc aaaacgattg ccgaattacc gctttttctc acagtgccac
     3421 tggtcttcac ggcgattgcc tatccgatga tcggactgcg ggccggagtg ctgcacttct
     3481 tcaactgcct ggcgctggtc actctggtgg ccaatgtgtc aacgtccttc ggatatctaa
     3541 tatcctgcgc cagctcctcg acctcgatgg cgctgtctgt gggtccgccg gttatcatac
     3601 cattcctgct ctttggcggc ttcttcttga actcgggctc ggtgccagta tacctcaaat
     3661 ggttgtcgta cctctcatgg ttccgttacg ccaacgaggg tctgctgatt aaccaatggg
     3721 cggacgtgga gccgggcgaa attagctgca catcgtcgaa caccacgtgc cccagttcgg
     3781 gcaaggtcat cctggagacg cttaacttct ccgccgccga tctgccgctg gactacgtgg
     3841 gtctggccat tctcatcgtg agcttccggg tgctcgcata tctggctcta agacttcggg
     3901 cccgacgcaa ggagtagccg acatatatcc gaaataactg cttgtttttt tttttaccat
     3961 tattaccatc gtgtttactg tttattgccc cctcaaaaag ctaatgtaat tatatttgtg
     4021 ccaataaaaa caagatatga cctatagaat acaagtattt ccccttcgaa catccccaca
     4081 agtagacttt ggatttgtct tctaaccaaa agacttacac acctgcatac cttacatcaa
     4141 aaactcgttt atcgctacat aaaacaccgg gatatatttt ttatatacat acttttcaaa
     4201 tcgcgcgccc tcttcataat tcacctccac cacaccacgt ttcgtagttg ctctttcgct
     4261 gtctcccacc cgctctccgc aacacattca ccttttgttc gacgaccttg gagcgactgt
     4321 cgttagttcc gcgcgattcg gttcgctcaa atggttccga gtggttcatt tcgtctcaat
     4381 agaaattagt aataaatatt tgtatgtaca atttatttgc tccaatatat ttgtatatat
     4441 ttccctcaca gctatattta ttctaattta atattatgac tttttaaggt aattttttgt
     4501 gacctgttcg gagtgattag cgttacaatt tgaactgaaa gtgacatcca gtgtttgttc
     4561 cttgtgtaga tgcagtcgcg ctcgcgcgac tgacggtcgt aagcacccgc gtacgtgtcc
     4621 accccggtca caaccccttg tgtcatgtcg gcgaccctac gcccccaact gagagaactc
     4681 aaaggttacc ccagttgggg cactactccc gaaaaccgct tctgacctgg gaaaacgtga
     4741 agccccgggg catccgctga gggttgccgc cggggcttcg gtgtgtccgt cagtactgca
     4801 tctcaaaaaa atggtgggca taatagtgtt gtttatatat atcaaaaata acaactataa
     4861 taataagaat acatttaatt tagaaaatgc ttggatttca ctggaactag aattaattcg
     4921 gctgctgctc taaacgacgc atttcgtact ccaaagtacg aattttttcc ctcaagctct
     4981 tattttcatt aaacaatgaa caggacctaa cgcacagtca cgttattgtt tacataaatg
     5041 atttttttta ctattcaaac ttactctgtt tgtgtactcc cactggtata gccttctttt
     5101 atcttttctg gttcaggctc tatcacttta ctaggtacgg catctgcgtt gagtcgcctc
     5161 cttttaaatg tctgaccttt tgcaggtgca gccttccact gcgaatcatt aaagtgggta
     5221 tcacaaattt gggagttttc accaaggctg cacccaaggc tctgctccca caattttctc
     5281 ttaatagcac acttcggcac gtgaattaat tttactccag tcacagcttt gcagcaaaat
     5341 ttgcaatatt tcattttttt ttattccacg taagggttaa tgttttcaaa aaaaaattcg
     5401 tccgcacaca acctttcctc tcaacaagca aacgtgcact gaatttaagt gtatacttcg
     5461 gtaagcttcg gctatcgacg ggaccacctt atgttatttc atcatgggcc agacccacgt
     5521 agtccagcgg cagatcggcg gcggagaagt taagcgtctc caggatgacc ttgcccgaac
     5581 tggggcacgt ggtgttcgac gatgtgcagc taatttcgcc cggctccacg tccgcccatt
     5641 ggttaatcag cagaccctcg ttggcgtaac ggaaccatga gaggtacgac aaccatttga
     5701 ggtatactgg caccgagccc gagttcaaga agaaggcgtt tttccatagg ctccgccccc
     5761 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     5821 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5881 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     5941 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6001 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6061 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6121 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6181 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6241 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6301 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6361 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6421 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6481 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6541 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6601 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     6661 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6721 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6781 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     6841 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6901 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6961 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7021 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7081 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
     7141 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7201 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7261 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7321 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7381 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7441 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     7501 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     7561 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     7621 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     7681 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     7741 accatatgcg gtgtgaaata ccgcaccgaa tcgcgcggaa ctaacgacag tcgctccaag
     7801 gtcgtcgaac aaaaggtgaa tgtgttgcgg agagcgggtg ggagacagcg aaagagcaac
     7861 tacgaaacgt ggtgtggtgg aggtgaatta tgaagagggc gcgcgatttg aaaagtatgt
     7921 atataaaaaa tatatcccgg tgttttatgt agcgataaac gagtttttga tgtaaggtat
     7981 gcaggtgtgt aagtcttttg gttagaagac aaatccaaag tctacttgtg gggatgttcg
     8041 aaggggaaat acttgtattc tataggtcat atcttgtttt tattggcaca aatataatta
     8101 cattagcttt ttgagggggc aataaacagt aaacacgatg gtaataatgg taaaaaaaaa
     8161 aacaagcagt tatttcggat atatgtcggc tactccttgc gtcgggcccg aagtcttaga
     8221 gccagatatg cgagcacccg gaagctcacg atgagaatgg ccagac
//
